Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization
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چکیده
منابع مشابه
Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization.
Suppression subtractive hybridization (SSH) is one of the most powerful and popular methods for isolating differentially expressed transcripts. However, SSH-generated libraries typically contain some background clones representing non-differentially expressed transcripts. To overcome this problem we developed a simple procedure that substantially decreases the number of background clones. This ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.20.e90